Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Hydrogenobacter thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
reduced ferredoxin 1 | at pH 8.0 and 70°C | Hydrogenobacter thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | - |
- |
- |
Hydrogenobacter thermophilus TK-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
butyl-Toyopearl column chromatography, DEAE-Toyopearl column chromatography, hydroxyapatite column chromatography and MonoQ column chromatography | Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.019 | - |
crude extract, with ferredoxin 1 as electron donor, at pH 8.0 and 70°C | Hydrogenobacter thermophilus |
14 | - |
after 758fold purification, with ferredoxin 1 as electron donor, at pH 8.0 and 70°C | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no significant nitrate-reducing activity is detected when NADH or NADPH is used as the electron donor. The enzyme also shows no significant reactivity toward methylviologen, benzyl viologen or ferredoxin 3 | Hydrogenobacter thermophilus | ? | - |
- |
|
additional information | no significant nitrate-reducing activity is detected when NADH or NADPH is used as the electron donor. The enzyme also shows no significant reactivity toward methylviologen, benzyl viologen or ferredoxin 3 | Hydrogenobacter thermophilus TK-6 | ? | - |
- |
|
nitrate + 2 reduced ferredoxin 1 + 2 H+ | - |
Hydrogenobacter thermophilus | nitrite + H2O + 2 oxidized ferredoxin 1 | - |
? | |
nitrate + 2 reduced ferredoxin 1 + 2 H+ | - |
Hydrogenobacter thermophilus TK-6 | nitrite + H2O + 2 oxidized ferredoxin 1 | - |
? | |
nitrate + 2 reduced ferredoxin 2 + 2 H+ | - |
Hydrogenobacter thermophilus | nitrite + H2O + 2 oxidized ferredoxin 2 | - |
? | |
nitrate + 2 reduced ferredoxin 2 + 2 H+ | - |
Hydrogenobacter thermophilus TK-6 | nitrite + H2O + 2 oxidized ferredoxin 2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77000, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
Assimilatory nitrate reductase | - |
Hydrogenobacter thermophilus |
Fd-NAS | - |
Hydrogenobacter thermophilus |
NAS | - |
Hydrogenobacter thermophilus |
NasB | - |
Hydrogenobacter thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.4 | the enzyme has high activity between pH 7.5 and 8.4 | Hydrogenobacter thermophilus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is necessary for nitrate assimilation | Hydrogenobacter thermophilus |